中国炼油与石油化工 ›› 2022, Vol. 24 ›› Issue (1): 129-134.

• Environmental Protection • 上一篇    下一篇

Investigation of nitrite production pathway in integrated partial denitrification/anammox process via isotope labelling technique and the relevant microbial communities

李妍喆1,盖佳柠1,张晓飞2,赵东风3,国亚东4,余庚星5,赵朝成1,刘芳6,赵瑞玉1,刘春爽1   

  1. 1. 中国石油大学(华东)
    2. 中国石油集团安全环保技术研究院有限公司
    3. 中国石油大学(华东)环境与安全工程系
    4. 中国石油大学(华东)化学工程学院
    5. 哈尔滨建实科技有限公司
    6. 中国石油大学化工学院
  • 收稿日期:2021-07-29 修回日期:2021-08-31 出版日期:2022-03-30 发布日期:2022-03-30
  • 通讯作者: 刘春爽 E-mail:liuchunshuang723@126.com
  • 作者简介:2021-12-31
  • 基金资助:
    国家自然科学基金;山东省自然科学基金;山东省重点研发计划重大科技创新工程;中央高校科研业务费

Investigation of nitrite production pathway in integrated partial denitrification/anammox process via isotope labelling technique and the relevant microbial communities

Yan-Zhe LI1, Zhao Dongfeng3,Guo Yadong3, Zhao Chaocheng3,Liu Fang3, Liu Chunshuang4   

  • Received:2021-07-29 Revised:2021-08-31 Online:2022-03-30 Published:2022-03-30
  • Contact: Liu Chunshuang E-mail:liuchunshuang723@126.com

摘要: 本文采用UASB反应器研究了短程反硝化/厌氧氨氧化(PDA)工艺的脱氮性能。在氮负荷率为0.64kg/(m3?d)实现了较高的总氮(TN)去除率(91.97%)。15N同位素标记实验结果显示,厌氧氨氧化菌确实在PDA中执行了将硝酸盐转化为亚硝酸盐的功能,贡献率约为36.3%。Candidatus_Brocadia, Candidatus_Kuenenia 和Thauera分别是厌氧氨氧化和反硝化过程的功能菌株。Thauera 和Candidatus_Brocadia 在高负荷下(0.64kg/(m3?d))对TN去除非常重要。实验结果为PDA工艺的应用提供了理论和技术基础。

关键词: 短程反硝化, 厌氧氨氧话, 15N同位素实验, 生物脱氮

Abstract: In this study, the nitrogen removal performance of PDA process was investigated by using UASB reactor. High TN removal efficiency (91.97%) was achieved at influent nitrogen loading rate of 0.64 kg/m3d. Anammox bacteria did execute the function of converting nitrate to nitrite in PDA system according to 15N isotope labeling experiments and the contribution was approximately 36.3%. Candidatus_Brocadia, Candidatus_Kuenenia and Thauera were functional strains for anammox and denitrification process, respectively. Thauera and Candidatus_Brocadia were more important for TN removal at high loading rates (0.64 kg/m3d). This result provides a theoretical and technical foundation for the application of denitrifying ammonium oxidation process.