中国炼油与石油化工 ›› 2014, Vol. 16 ›› Issue (01): 19-26.

• Scientific Research • 上一篇    下一篇

响应曲面法优化假单胞菌LKY-5对二苯并噻吩的降解

李琳1,赵朝成1,刘其友1,张云波1,刘春爽1,薛建良2   

  1. 1. 中国石油大学(华东)
    2. 山东科技大学化学与环境工程学院环境科学与工程系
  • 收稿日期:2013-11-18 修回日期:2013-12-22 出版日期:2014-03-30 发布日期:2014-03-30
  • 通讯作者: 赵朝成 E-mail:zhaochch@upc.edu.cn
  • 基金资助:
    中央高校基本科研业务费专项资金

Optimization for the Microbial Degradation of Dibenzothiophene by Pseudomonas sp. LKY-5 using Response Surface Methodology

Li Lin,Zhao Chaocheng,Liu Qiyou,Zhang Yunbo,Liu Chunshuang,Xue Jianliang   

  • Received:2013-11-18 Revised:2013-12-22 Online:2014-03-30 Published:2014-03-30
  • Contact: Zhao Chaocheng E-mail:zhaochch@upc.edu.cn
  • Supported by:
    the Fundamental Research Funds for the Central Universities

摘要: 本文选择已从石油污染土壤中分离得到的假单胞菌LKY-5进行二苯并噻吩的微生物降解研究。基于Box–Behnken设计的响应曲面法被用来分析四个独立因素对微生物降解效果的影响。这四个因素分别是底物浓度、温度、pH和摇床转速。本研究进行了四因素三水平共计29组实验,并得到了一个有效的二阶回归模型。方差分析表明该二阶模型的预测值与实验值有很好的符合性(R2=0.9534)。实验结果发现底物浓度对二苯并噻吩的降解效果的影响最为显著,当底物浓度为100mg/L时,假单胞菌LKY-5的降解率能达到100%。

关键词: 降解, 二苯并噻吩, 假单胞菌, 响应曲面法, Box–Behnken设计

Abstract: Abstract: In this research, the degradation of dibenzothiophene (DBT) was investigated by Pseudomonas sp. LKY-5 isolated from oil contaminated soil. The response surface methodology (RSM) based on Box–Behnken design (BBD) was applied for evaluating the interactive effects of four independent variables including substrate concentration, temperature, pH and agitation rate on the DBT removal response. A total of 29 experiments for four factors at three levels were conducted in present study. A second-order regression model was then developed, and the analysis of variance (ANOVA) illustrated that the proposed quadratic model could be utilized to navigate the design space. The value of determination coefficient (R2=0.9534) indicated a satisfactory agreement between the quadratic model and the experimental data. It was found that DBT removal was more significantly affected (P<0.0001) by substrate concentration compared with other three parameters. The 100% degradation of DBT could be obtained by Pseudomonas sp. LKY-5 at substrate concentration of 100 mg/L.

Key words: degradation, dibenzothiophene, Pseudomonas sp., response surface methodology, Box–Behnken design